Thermo Fisher Scientific AKAP8 Recombinant Rabbit Monoclonal Antibody (JE63-83)
AKAP8 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC 등 다양한 응용에 사용 가능. HEK293 발현 시스템에서 생산된 recombinant IgG 항체이며, Protein A 정제. 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JE63-83 |
| Immunogen | Recombinant protein within human AKAP 95 aa 1-150/692 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 0.05% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2931246 |
Target Information
The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is mediated by compartmentalization of the kinase to specific sites within the cell. The R subunit (RII) of PKA interacts with RII-anchoring proteins, collectively known as A-kinase anchoring proteins (AKAPs).
AKAP 95, also known as AKAP8, is a nuclear matrix protein predominantly expressed in liver, heart, pancreas, kidney, and skeletal muscle. During mitosis, AKAP 95 is recruited to chromosomes and plays an essential role in mitotic progression. It participates in PKA signaling through interaction with the RII regulatory subunit and forms a complex with HA95 and HDAC3, required for deacetylation of Histone H3 during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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