Thermo Fisher Scientific CHCHD6 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human CHCHD6. Recombinant protein control fragment (Product #RP-100169). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CHCHD6,uniProtId:Q9BRQ6-1,ncbiNodeId:9606,antigenRange:2-71,antigenLength:235,antigenImageFileName:PA5-61567_CHCHD6_Q9BRQ6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-61567_CHCHD6_Q9BRQ6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2639753

Product Specific Information

Immunogen sequence: GSTESSEGRR VSFGVDEEER VRVLQGVRLS ENVVNRMKEP SSPPPAPTSS TFGLQDGNLR APHKESTLPR

Highest antigen sequence identity to the following orthologs: Mouse - 47%, Rat - 47%.

Target Information

CHCHD6 is a member of a family of proteins containing a conserved (coiled coil 1)-(helix 1)-(coiled coil 2)-(helix 2) domain and has been observed in a complex with the mitochondrial proteins mitofilin, SAM50, metaxins 1 and 2, and CHCHD3. CHCHD6 knockdown causes severe defects in mitochondrial cristae morphology as well as reductions in cell growth, ATP production and oxygen consumption. This decrease of CHCHD6 also led to a reduction in mitofilin protein levels, while a knockdown of mitofilin resulted in a reduced level of CHCHD6, suggesting coordinate regulation between these two proteins. CHCHD6 knockdown in human cancer cells enhances their sensitivity to genotoxic anticancer drugs, suggesting that CHCHD6 may be a potential therapeutic anti-tumor target.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.