Thermo Fisher Scientific TIMM50 Recombinant Rabbit Monoclonal Antibody (JE51-61)
Recombinant rabbit monoclonal antibody against human TIMM50. Validated for WB and IHC-P. Expressed in HEK293 cells and purified via Protein A. Supplied as a liquid at 1 mg/mL, suitable for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JE51-61 |
| Immunogen | Recombinant protein within Human TIM50 aa 1–109 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 0.05% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_2848789 |
Product Specific Information
Positive Control: A549, K562, MCF-7, human liver carcinoma tissue, human colon carcinoma tissue, human breast carcinoma tissue.
Target Information
TIMM50 (Mitochondrial import inner membrane translocase subunit TIM50) is an enzyme encoded by the TIMM50 gene in humans. Tim50 is a subunit of the Tim23 translocase complex located in the inner mitochondrial membrane. Mutations in TIMM50 have been associated with epilepsy, severe intellectual disability, and 3-methylglutaconic aciduria. Expression of TIMM50 is increased in breast cancer cells and decreased in hypertrophic hearts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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