Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (YKIX753.22), PE, eBioscience
개 조혈세포 표지자인 CD45R을 인식하는 YKIX753.22 단클론 항체로, PE 형광체 결합형입니다. 유세포분석용으로 최적화되어 있으며, 4°C 암소 보관 권장. 높은 특이성과 재현성으로 B세포 및 T세포 분석에 적합.
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Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (YKIX753.22), PE, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.0075 µg)/test
- Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog |
| Published Species | Dog |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | YKIX753.22 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10597010 |
Product Specific Information
The YKIX753.22 monoclonal antibody reacts with canine CD45R.
In dogs, CD45R is expressed on B cells and peripheral T cells. CD45 exists as multiple isoforms (CD45RA, CD45RB, CD45RO) generated by alternative splicing. The C-terminal domain of CD45 exhibits tyrosine phosphatase activity, enabling signaling from T and B cell receptors following activation.
This antibody can promote cell depletion via antibody-dependent cell-mediated cytotoxicity when used with YKIX716.13.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and validated on normal canine peripheral blood cells
- Recommended Use: 5 µL (0.0075 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family, which regulates cellular processes including growth, differentiation, mitosis, and oncogenic transformation.
CD45 contains an extracellular domain, a transmembrane segment, and two intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and is essential for T- and B-cell receptor signaling.
CD45 glycoprotein plays a critical role in lymphocyte development and antigen signaling, regulating Src-family kinases. Isoforms generated by alternative splicing differ in extracellular domains but share identical transmembrane and cytoplasmic domains. Expression varies by cell type and physiological state.
Additionally, CD45 modulates integrin-mediated signal transduction and is involved in apoptosis-related DNA fragmentation.
CD45RA isoform shows restricted expression among lymphoid cell subtypes.
Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency and Hepatitis C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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