Thermo Fisher Scientific CellSensor ESRE-bla HeLa Cell Line

Thermo Fisher Scientific CellSensor™ ESRE-bla HeLa Cell Line

Endoplasmic Reticulum (ER) stress is associated with various pathophysiological conditions such as neurodegenerative diseases, diabetes, tumor growth under hypoxic conditions, and ischemic heart disease. Under stress conditions, proteins in the ER misfold or unfold and accumulate, promoting the expression of ER stress-responsive genes.

One of the mechanisms mediating ER stress response is the activation of transcription factor ATF6. The quiescent form of ATF6 (p90ATF6), a type II transmembrane protein, resides in the ER membrane and is proteolyzed in an ER stress-dependent manner. The liberated N-terminal fragment (p50ATF6) translocates to the nucleus and binds to the ER stress response element (ERSE) present in promoter regions of many ER stress-responsive proteins, including ER chaperones.

To better understand these pathological processes and explore potential therapies, ESRE–bla HeLa cells are engineered to express beta-lactamase under the control of the ER stress response element. This clonal population was isolated by FACS, and dose-response curves with tunicamycin and thapsigargin have been performed. The cell line also responds to other known ER stress inducers.

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