Thermo Fisher Scientific DWARF 14 Polyclonal Antibody
식물 D14 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P) 분석에 적합. Strigolactone 신호전달 관련 연구에 활용 가능. 고순도 친화 크로마토그래피 정제, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Plant |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within N-terminal Rice D14 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2913259 |
Product Specific Information
- Positive Control: Rice
- Subcellular Location: Cytoplasm, Nucleus
Target Information
This antibody recognizes the DWARF 14 (D14) protein involved in the strigolactone (SL) signaling pathway. D14 may act downstream of SL synthesis as part of hormone signaling or as an enzyme that converts SL into its bioactive form.
Strigolactones are plant hormones that:
- Inhibit tillering and shoot branching via the MAX-dependent pathway
- Regulate shoot architecture under phosphate-limiting conditions
- Function as rhizosphere signals stimulating hyphal branching of arbuscular mycorrhizal fungi
- Trigger seed germination of root parasitic weeds
SL-dependent association of D14 with D3 and D53 leads to D53 ubiquitination and degradation. D14 hydrolyzes the butenolide ring of SLs, forming D-OH, which induces interaction with SLR1 and possibly other proteins such as D3 and D53. D14 also contributes to the negative regulation of gibberellin signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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