Thermo Scientific™

DTT (dithiothreitol)

Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It자세히 알아보기

Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro. DTT quantitatively reduces disulfide bonds and maintains monothiols in a reduced state (see Reference 1).

At a final 0.1 M concentration, DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis.

Highlights

• Free of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases.

Applications

• Enzyme stabilization
• Enzymatic reactions
• Protein electrophoresis

Note
• DTT is not stable in solution. Only freshly-made DTT solutions should be used. To prepare a 1 M DTT solution, dissolve 1.55 g of DTT powder in 10 mL of deionized water (e.g. Water, nuclease-free, #R0581).

사양

농도0.1 M

제품 유형DTT (Dithiothreitol)

수량5 g

Unit SizeEach