Thermo Fisher Scientific MTBP Monoclonal Antibody (PHL-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:2,000

-

ELISA (ELISA)

1:2,000-1:20,000

-

Product Specifications

Species Reactivity

Chimpanzee, Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

PHL-1

Immunogen

recombinant amino-terminal HIS6X-tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MTBP,uniProtId:Q96DY7-1,ncbiNodeId:9606,antigenRange:667-812,antigenLength:904,antigenImageFileName:500-301-875_MTBP_Q96DY7-1_House_mouse.svg,antigenImageFileNamePDP:500-301-875_MTBP_Q96DY7-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

0.02M potassium phosphate/ascites, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is directed against human MTBP protein. The product was produced from clarified mouse ascetic fluid. A BLAST analysis was used to suggest reactivity with this protein from human (100% homology) and chimpanzee. Cross reactivity with MTBP protein from mouse (77% homology) and chicken (79% homology) is unlikely due to the relatively low number of positive residues and high number of gaps within this sequence. Cross reactivity with MTBP homologues from other sources has not been determined.

Target Information

MTBP inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells. The protein may play a role in MDM2-dependent TP53/p53 homeostasis in unstressed cells. The protein inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of TP53/p53 and its subsequent degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.