Applied Biosystems™

MethylCode™ Bisulfite Conversion Kit

The MethylCode™ Bisulfite Conversion Kit is a fast and easy way to convert unmethylated cytosines from a DNA sample into자세히 알아보기

The MethylCode™ Bisulfite Conversion Kit is a fast and easy way to convert unmethylated cytosines from a DNA sample into uracils while methylated cytosines remain unchanged.

The modified DNA is ideal for PCR amplification for downstream analyses, including restriction endonuclease digestion, sequencing, and microarrays. With the MethylCode™ Bisulfite Conversion Kit you can expect:

–Simple, rapid cytosine methylation detection

–Consistency across most experimental conditions
–Streamlined conversion of unmethylated cytosines into uracil, thanks to a coupled DNA denaturation/bisulfite treatment step
–Ultra-pure DNA ideal for subsequent molecular-based analyses

**Kit specifications:

**–Protocol: Rapid heat denaturation of DNA followed by sodium bisulfite modification with spin column purification–Sample sources: Plasmid, genomic, and endonuclease digested DNAs
–Input DNA: 500 pg-2 μg DNA per treatment, with 200-500 ng being optimal–Recovery volume: Purified, bisulfite-treated DNA is recovered in 10 μl elution buffer

*Contents and storage:
*The MethylCode™ Bisulfite Conversion Kit contains all the reagents necessary to convert your DNA for methylation studies. The kit includes CT Conversion Reagent, Dilution Buffer, Resuspension buffer, Binding Buffer, Wash Buffer, Desulphonation Buffer, Elution Buffer, Spin Columns, Collection Tubes.

Store at room temperature for up to 24 months.

사양

제품 유형Kit, Bisulfite Conversion

샘플 종류DNA (Genomic)

배송 조건Room Temperature

시작 물질 셀 수Purified genomic DNA - 200 ng to 500 ng

형식Kit

제품라인MethylCode™

수량50 Reactions

Unit SizeEach