Thermo Scientific™

Pierce™ Biotin 3` End DNA Labeling Kit

The Thermo Scientific Pierce Biotin 3` End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use자세히 알아보기

The Thermo Scientific Pierce Biotin 3` End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.

The DNA biotinylation procedure uses terminal deoxynucleotidyl transferase (TdT) to catalyze nontemplate-directed nucleotide incorporation onto the 3-OH end of single-stranded DNA. TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3 overhangs and blunt duplexes, albeit with a lower efficiency.

Features of the Biotin 3` End DNA Labeling Kit:

• Non-isotopic labeling eliminates the hassle of hazardous radioactive materials or difficult-to-dispose-of waste
• 1-3 biotinylated ribonucleotides onto the 3` end of DNA strands for less interference with hybridization or sequence-specific binding of proteins
• Biotin-labeled probes are stable for more than one year
• 30-minute labeling procedure is fast and efficient

The Biotin 3End DNA Labeling Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (Biotin-11-UTP) onto the 3 end of DNA strands. This labeling strategy has the advantage of localizing the biotin to the 3` end of the probe where it will be less likely to interfere with hybridization or sequence-specific binding of proteins. Biotin-labeled DNA probes can be used to facilitate non-isotopic detection in a variety of applications including electrophoretic mobility shift assays (EMSA), Northern or Southern blots, colony hybridizations or in situ hybridizations.

사양

형식Kit

Labeling TargetDNA

반응 수20

제품라인Pierce™

제품 유형DNA Labeling Kit

수량20 Reactions

Unit SizeEach