Thermo Fisher Scientific CD15/FUT4 (Reed-Sternberg Cell Marker) Monoclonal Antibody (SPM490)
인간 CD15/FUT4 항원을 인식하는 단클론 항체로 Reed-Sternberg 세포 마커로 사용됨. IHC 및 ICC/IF 등 다양한 면역염색 응용에 적합. 단클론 Mouse IgM 형식으로 1 mg/mL 농도, PBS 버퍼에 보존. 연구용으로만 사용 가능.
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Thermo Fisher Scientific CD15/FUT4 (Reed-Sternberg Cell Marker) Monoclonal Antibody (SPM490)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM490 |
| Immunogen | Myelomonocytic leukemia cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins, and proteoglycans of various cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and various tumor cells.
Variants of Lewis X include sialyl-Lewis X and sulphated Lewis X. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation via calcium-dependent Le(x)-Le(x) interactions, which play roles in embryo compaction and teratocarcinoma cell autoaggregation.
Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been widely used to confirm diagnoses of Hodgkin’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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