pJTI™ R4 CMV-TO MCS pA Vector

The pJTI™ R4 CMV-TO MCS pA vector is designed for the expression of your gene of interest under the control자세히 알아보기

The pJTI™ R4 CMV-TO MCS pA vector is designed for the expression of your gene of interest under the control of the tet-inducible CMV promoter after restriction enzyme cloning and retargeting into the genomic R4 site of a Jump-In™ parental cell line.

This vector can be used for inducible or constitutive expression of your gene of interest, depending on which Jump-In™ parental cell line you use. When this vector is retargeted into a Jump-In™ T-REx™ parental cell line, gene expression is controlled by the tet-operon and can be induced by adding doxycycline to the growth media. When used with a Jump-In™ parental cell line such as the Jump-In™ GripTite™ HEK293 cell line, constitutive expression is achieved after retargeting. The R4 sites in the Jump-In™ parental cell lines result in a high retargeting efficiency, requiring less effort and less time than traditional cell engineering methods. Retargeting of Jump-In™ parental cell lines results in creation of an isogenic pool that is sufficient for cell-based experiments without the need for clonal selection. Alternatively, the high retargeting efficiency allows for easy selection of a positive stable clone for expressing your gene of interest.

사양

구성 또는 유도성 시스템Inducible

배달 유형Transfection

유도제Tetracycline

프로모터CMV/TO

제품 유형Mammalian Expression Vector

선택 제제(진핵)Geneticin™ (G-418)

항생제 내성 박테리아Ampicillin (AmpR)

단백질 태그Untagged

클로닝 방법Restriction Enzyme/MCS

수량100 μg

벡터Jump-In Vectors

제품라인Jump-In™

용도(애플리케이션)Stable Cell Line Development, Targeted Integration, Regulated Expression

Unit SizeEach