Thermo Fisher Scientific pJTI R4 CMV-TO MCS pA Vector

Thermo Fisher Scientific pJTI™ R4 CMV-TO MCS pA Vector

The pJTI™ R4 CMV-TO MCS pA vector is designed for the expression of your gene of interest under the control of the tet-inducible CMV promoter after restriction enzyme cloning and retargeting into the genomic R4 site of a Jump-In™ parental cell line.

This vector can be used for inducible or constitutive expression of your gene of interest, depending on which Jump-In™ parental cell line you use.

• When retargeted into a Jump-In™ T-REx™ parental cell line, gene expression is controlled by the tet-operon and can be induced by adding doxycycline to the growth media.
• When used with a Jump-In™ GripTite™ HEK293 cell line, constitutive expression is achieved after retargeting.

The R4 sites in the Jump-In™ parental cell lines enable high retargeting efficiency, reducing time and effort compared to traditional cell engineering methods. Retargeting results in an isogenic pool suitable for cell-based experiments without clonal selection, or allows easy selection of a stable clone expressing your gene of interest.

사양 (Specifications)