Invitrogen™

One Shot™ INV110 Chemically Competent E. coli

One Shot INV110 Chemically Competent E. coli cells are derived from the JM110 strain and are specifically designed for the자세히 알아보기

One Shot INV110 Chemically Competent E. coli cells are derived from the JM110 strain and are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. The dam and dcm deficiency allows for the production of plasmid DNA that is unmethylated at GmATC and CmC(A/T)GG sites. INV110 Chemically Competent cells can achieve a transformation efficiency of >1 x 106 cfu/μg of control DNA.

The INV110 strain is a significant improvement over the JM110 strain. The INV110 strain has been engineered to carry an end_A1 mutation, which eliminates the non-specific Endonuclease I, for improved plasmid DNA preparation. Mutation in the methylation-dependent restriction system Δ(_mcr_C-_mrr) allows transformation of genomic DNA. This strain also has the lac_ZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. The INV110 strain contains an F´ episome that can support cloning plasmids with an f1-like origin of replication (phagemids) and allows isolation of single-stranded DNA (ssDNA). In addition, the F´ episome carries the _lac_Iq repressor for inducible expression from _trc, tac, and lac promoters using IPTG.

One Shot INV110 Chemically Competent E. coli offer:
• Transformation efficiencies of >1 x 106 cfu/μg
• dam and dcm deficiency to allow restriction digestion with dam and dcm-sensitive restriction enzymes
• Stable F´ episome that allows isolation of ssDNA and carries lac_Iq repressor for inducible expression from _trc, tac, and lac promoters
• Δ(mcr_C-_mrr) mutation that reduces cleavage of foreign methylated DNA and improves transformation of genomic DNA
• _lac_ZΔM15 for blue-white color screening of recombinant clones
• _end_A1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• Tn10 to permit selection of the host strain using tetracycline

Note: dam deletion results in higher mutation rate, higher recombination frequency, and lower viability on plates. Avoid picking colonies that are noticeably larger than the rest of the population (potential revertants). Pick colonies and isolate the DNA within one day of transformation.

Convenient and efficient format
One Shot INV110 Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation, reducing both freeze-thaw cycles and costs associated with unused cells.

Genotype
F´ [tra_D36 _pro_AB _lac_Iq_lac_ZΔM15] _rps_L (StrR) _thr leu end_A _thi_-1 _lac_Y _gal_K _gal_T _ara ton_A _tsx dam dcm sup_E44 Δ(_lac_-_pro_AB) Δ(_mcr_C-_mrr)102::_Tn_10(TetR)

Find the strain and format that fits your needs
We offer other strains in chemically competent and electrocompetent cell formats to meet your specific needs.
Strains in MultiShot formats for high throughput applications are available.
Alternative strains for ssDNA production are available, such as TOP10F´ or electrocompetent DH12S.
Explore bacterial growth media formats.
Explore restriction enzyme details to find methylation sensitivity.

사양

제품 유형Chemically Competent Cells

에프에피솜 포함Yes

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝Yes (mcr, mrr)

형질전환 효율 수준Subcloning Efficiency (1 x 106 to 1 x 107 cfu/μg)

항생제 내성 박테리아Yes (Streptamycin, Tetracycline)

불안정 DNA 클로닝No

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

비메틸화 DNA 준비No

재조합 감소No

배송 조건Dry Ice

T1 Phage - 저항성(tonA)Yes

종E. coli (K12)

형식Tube

제품라인One Shot™

수량21 x 50 μL/tube

Unit SizeEach