Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugate Formats

• Alexa Fluor 488, 532, 561, 647, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 563, 737, 805
• Brilliant Violet 421, 711, 786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue / Red / UV / Violet / Yellow Series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132 (common gamma chain, γc) to form the IL-7 receptor (IL-7R).
CD127 is a type I glycoprotein (75–80 kDa) expressed by immature B cells, thymocytes, and most mature T cells, with transient down-regulation upon activation.

Binding of IL-7 triggers signal transduction through tyrosine kinase pathways, including Jak/STAT. IL-7 is essential for lymphocyte development and T-cell homeostasis.

CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (Tregs), making CD127 a useful marker for identifying true Treg populations enriched for Foxp3 expression and suppressive activity.

Binding of eBioRDR5 to PBMCs is blocked by pre-incubation with recombinant human IL-7 (Product # 14-1079-80).

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on normal human peripheral blood cells at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD127 (IL-7Rα) is a glycoprotein involved in lymphopoiesis regulation. The IL-7 receptor is an α/γ heterodimer; the γ chain activates signal transduction, while the α chain mediates specific signaling events.
CD127 supports proliferation of precursor B cells, thymocytes, T-cell progenitors, and mature CD4+/CD8+ T cells. Dysfunction is associated with severe combined immunodeficiency (SCID).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.