Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), eBioscience
인간 CD127(IL-7Rα)을 인식하는 eBioRDR5 단클론 항체로, Flow Cytometry 및 IHC에 사용 가능. IL-7 신호전달 및 조절 T세포 연구에 적합. 고순도(>90%)로 제조되어 안정적이며 다양한 형광 및 효소 결합 형식으로 제공.
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- 14-1278-82
- 판매단위
- pk
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Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 24 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioRDR5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657591 |
Available Conjugate Formats
- Alexa Fluor 488, 532, 561, 647, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 496, 563, 737, 805
- Brilliant Violet 421, 711, 786
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue / Red / UV / Violet / Yellow Series
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132 (common gamma chain, γc) to form the IL-7 receptor (IL-7R).
CD127 is a type I glycoprotein (75–80 kDa) expressed by immature B cells, thymocytes, and most mature T cells, with transient down-regulation upon activation.
Binding of IL-7 triggers signal transduction through tyrosine kinase pathways, including Jak/STAT. IL-7 is essential for lymphocyte development and T-cell homeostasis.
CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (Tregs), making CD127 a useful marker for identifying true Treg populations enriched for Foxp3 expression and suppressive activity.
Binding of eBioRDR5 to PBMCs is blocked by pre-incubation with recombinant human IL-7 (Product # 14-1079-80).
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on normal human peripheral blood cells at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD127 (IL-7Rα) is a glycoprotein involved in lymphopoiesis regulation. The IL-7 receptor is an α/γ heterodimer; the γ chain activates signal transduction, while the α chain mediates specific signaling events.
CD127 supports proliferation of precursor B cells, thymocytes, T-cell progenitors, and mature CD4+/CD8+ T cells. Dysfunction is associated with severe combined immunodeficiency (SCID).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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