
Thermo Fisher Scientific Phospho-ERK1/ERK2 (Thr202, Tyr204, Thr185, Tyr187) Recombinant Rabbit Monoclonal Antibody (23GB4505)
Phospho-ERK1/ERK2 (Thr202/Tyr204, Thr185/Tyr187)을 인식하는 재조합 토끼 단클론 항체로, Western blot과 유세포분석에 적합합니다. 인간, 마우스, 랫트 반응성이 있으며, 고순도 친화크로마토그래피 정제 및 안정한 PBS/glycerol buffer로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Flow Cytometry (Flow) | 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 23GB4505 |
| Immunogen | A synthesized peptide derived from human Phospho-ERK1 (Tyr204) + ERK2 (Tyr187) (195–211AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Dry ice (international) |
Target Information
ERK1 and ERK2 are widely expressed and play key roles in regulating meiosis, mitosis, and postmitotic functions in differentiated cells. These kinases are activated by various stimuli such as growth factors, cytokines, viral infection, and G protein-coupled receptor ligands. Activation occurs through the Ras–Raf–MEK–ERK signaling cascade, leading to phosphorylation of cytoplasmic and nuclear targets including transcription factors like c-Fos and Elk-1.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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