Thermo Fisher Scientific Pierce Rapid Antibody Isotyping Kit - Mouse

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카탈로그번호: 26178
브랜드: Thermo Fisher Scientific
카테고리: Antigen-Antibody Tests
판매단위: pk

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Thermo Fisher Scientific · Thermo Fisher Scientific Pierce Rapid Antibody Isotyping Kit - Mouse

Thermo Scientific™ Pierce™ Rapid Antibody Isotyping Kit - Mouse

The Thermo Scientific™ Pierce™ Rapid Mouse Antibody Isotyping Kit is a single-use, cassette-based kit for 5-minute lateral-flow assay determination of mouse monoclonal antibody class and subclass identity.

This kit provides a color-readout of the monoclonal antibody isotype within five minutes after pipetting a small amount of diluted culture supernatant or ascites fluid sample into the sample well. It determines mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM.

Features

Long shelf life: Stable for at least one year at room temperature
Single-step: Add diluted antibody sample to the cassette loading well
Fast: Band appears within 5 minutes indicating antibody isotype
Sensitive: Detects antibodies in tissue culture media or ascites fluid >10 ng/mL
Specific: No cross-reactivity with fetal bovine serum (FBS)
Reliable: Results comparable to ELISA-based isotyping assays

Description

Each assay is performed by adding a properly diluted tissue culture supernatant or mouse ascites sample to the cassette well. Gold conjugates form specific complexes with antibodies in the sample, which migrate along the membrane and are resolved on anti-isotype and class-specific antibody zones. Results are displayed as a red band indicating the antibody isotype or subclass.

Determining the class and subclass of a monoclonal antibody helps select the optimal purification method:

IgA, IgM: Purified by size exclusion or immunoaffinity columns
IgG2a, IgG2b: Purified with immobilized Protein A at pH 7–8
IgG1: Binds best to Protein A at pH 8–9
Kappa light chain antibodies: Purified using immobilized Protein L

The Pierce Rapid Isotyping Kit is compatible with both tissue culture supernatant and mouse ascites fluid. It is more sensitive than latex-bead dipstick assays and faster than ELISA-based assays.
Requires only 0.5 μL ascites fluid, 5–55 μL cell culture supernatant, or 1.5 ng purified antibody.

Specifications

항목	내용
설명	Pierce Rapid Isotyping Kit - Mouse
용도(애플리케이션)	Membrane Blot
수량	10 Tests
어세이	Isotyping Assay
검출 방법	Chromogenic
제품라인	Pierce™
타겟 특이도	Target-specific
Unit Size	Each