Thermo Fisher Scientific Histone H3 Polyclonal Antibody
Rabbit polyclonal antibody against Histone H3 for WB and IHC applications. Recognizes human, predicted reactive with mouse and chimpanzee. Supplied as liquid, 1 mg/mL, purified by antigen affinity chromatography. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 1–136 of Histone H3.1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552299 |
Product Specific Information
- Recommended positive control: A375
- Predicted reactivity: Mouse (100%), Chimpanzee (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it binds to DNA to form nucleosomes, the fundamental unit of chromatin structure. Histone H3 plays a crucial role in transcription regulation, DNA repair, replication, and chromosomal stability. The N-terminal tail of histone H3 undergoes various post-translational modifications, such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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