Thermo Fisher Scientific PHGDH Polyclonal Antibody
PHGDH 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC, ICC/IF에 적합하며 인간, 마우스, 랫트 시료에 반응. 항원 친화 크로마토그래피로 정제된 액상 항체로, 연구용으로 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 | — |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | — |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PHGDH. Recombinant protein control fragment (Product #RP-91732). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2645502 |
Product Specific Information
Immunogen sequence:
LEEIWPLCDF ITVHTPLLPS TTGLLNDNTF AQCKKGVRVV NCARGGIVDE GALLRALQSG QCAGAALDVF TEEPPRDRAL VDHENVISCP HLGASTKEAQ SRCGEEIAVQ FVDM
Highest antigen sequence identity to the following orthologs:
- Mouse: 99%
- Rat: 99%
Target Information
The PHGDH gene encodes an enzyme involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation, and other symptoms. Multiple alternatively spliced transcript variants have been identified, though the full-length nature of most is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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