Thermo Scientific™

Exonuclease III (200 U/μL)

Thermo Scientific Exonuclease III (ExoIII) exhibits four catalytic activities. The 3→5 exodeoxyribonuclease activity of ExoIII is specific for double-stranded DNA.자세히 알아보기

Thermo Scientific Exonuclease III (ExoIII) exhibits four catalytic activities. The 3→5 exodeoxyribonuclease activity of ExoIII is specific for double-stranded DNA. ExoIII degrades dsDNA from blunt ends, 5-overhangs or nicks, releases 5-mononucleotides from the 3-ends of DNA strands and produces stretches of single-stranded DNA. It is not active on 3-overhang ends of DNA that are at least four-bases long and do not carry a 3`-terminal C-residue on single-stranded DNA, or on phosphorothioate-linked nucleotides.

ExoIII 3-phosphatase activity removes the 3-terminal phosphate, generating a 3-OH group. ExoIII Rnase H activity exonucleolytically degrades the RNA strand in RNA-DNA hybrids. ExoIII apurinic/apyrimidinic-endonuclease activity cleaves phosphodiester bonds at apurinic or apyrimidinic sites to produce 5-termini that are base-free deoxyribose 5`-phosphate residues.

Highlights

• Active in restriction enzyme buffers

Applications

• Creation of unidirectional deletions in DNA fragments in conjunction with S1 Nuclease
• Generation of a single-stranded template for dideoxy-sequencing of DNA
• Site-directed mutagenesis
• Cloning of PCR products
• Preparation of strand-specific probes

Note

The rate of DNA digestion by ExoIII depends upon temperature, salt concentration, and the molar ratio of DNA to enzyme in the reaction mixture . Optimal reaction conditions should be determined experimentally.

사양

농도200 U/μL

효소Exonuclease

함께 사용가능한 버퍼Enzyme Buffer

수량4,000 units

제품 유형Exonuclease III

Unit SizeEach