Thermo Fisher Scientific GTPase HRAS Polyclonal Antibody, FITC
HRAS 단백질을 인식하는 FITC 결합 폴리클로날 항체로 Western blot, ELISA, Immunoprecipitation에 사용 가능. 인간, 생쥐, 돼지, 쥐, 초파리 반응성. 친화 크로마토그래피 정제 및 고감도 형광 검출용. 연구용으로만 사용.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly, Human, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 1–50 of human GTPase HRAS protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Monomeric G protein Ras functions as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to downstream signaling pathways, leading to distinct cytoplasmic or nuclear events.
Each mammalian cell contains three Ras proto-oncogenes coding for closely related Ras proteins: H-, K-, and N-Ras.
Oncogenic mutations in Ras genes are present in approximately 30% of all human cancers. Constitutive activation of Ras due to mutations or overexpression stimulates proliferation and inhibits apoptosis.
- K-Ras: Common in pancreatic, colorectal, and non-small-cell lung carcinomas
- H-Ras: Common in bladder, kidney, and thyroid carcinomas
- N-Ras: Found in melanoma, hematological malignancies, and hepatocellular carcinoma
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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