Thermo Scientific™

LanthaScreen™ TR-FRET VDR Coactivator Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15127:The LanthaScreen™ TR-FRET Vitamin D Receptor자세히 알아보기

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15127:

The LanthaScreen™ TR-FRET Vitamin D Receptor (VDR) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential VDR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a VDR ligand-binding domain (VDR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format.

*The assay
*To run the LanthaScreen™ TR-FRET VDR Coactivator Assay, VDR-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based upon the biology of the VDRcoactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).

*Contents and Storage:
*The LanthaScreen™ TR-FRET VDR Coactivator Assay Kit contains the VDR-LBD (GST) protein, fluorescein-labeled TRAP220/DRIP-2 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

사양

콘주게이트Tb (Terbium)

검출 방법Fluorescence

용도(장비)Microplate Reader

수량800 x 20 μL assays

포장384-well Plate

어세이 입력Biochemical coregulator interaction

유전자 ID(Entrez)7421

판독End Point

배송 조건Dry Ice

타겟 입력VDR, NR1I1

제품라인LanthaScreen™

용도(애플리케이션)Co-Factor Interaction Assay, TR-FRET

리간드VDR

제품 유형TR-FRET VDR Coactivator Assay Kit

No. of Assays800 x 20 μL Assays

Unit SizeEach