Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), NovaFluor Yellow 700, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), NovaFluor Yellow 700, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.2 µg/test

Product Specifications

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subset of mature T cells, and dendritic cells. CD4 binds to MHC class II molecules and plays a key role in T cell development and function. Binding of GK1.5 is blocked by RM4-5.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.2 µg/test (100 µL final volume). Cell count: 10⁵–10⁸ cells/test. Optimize antibody titration for best performance.

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
  → Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• Includes CellBlox Plus Blocking Buffer (Cat. No. C001T06F01). Add 5 µL per sample for optimal staining.
  For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).
• NovaFluor conjugates utilize Phiton™ technology for engineered fluorescent signatures minimizing spillover and spread.

Excitation: 552 nm
Emission: 700 nm
Laser: 561 nm (Yellow)

Note: Non-specific B cell staining observed in SJL mice; not in BALB/c or C57BL/6 strains.

Target Information

CD4 is a co-receptor for MHC class II molecules and HIV entry. Expressed mainly in T helper cells, but also in monocytes, macrophages, and dendritic cells. Found in thymus, lymph nodes, tonsils, spleen, and other tissues. CD4 assists in early T-cell activation and may mediate neuronal damage in immune-related CNS diseases. Multiple splice variants identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures.

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV/Violet/Yellow series
• eFluor 450, 506, 660
• FITC
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright series
• NovaFluor Blue/Red/Violet/Yellow variants
• Functional Grade
• Custom conjugation available

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