Thermo Fisher Scientific CNOT3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human CNOT3. Recombinant protein control fragment (Product #RP-89303). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CNOT3,uniProtId:O75175-1,ncbiNodeId:9606,antigenRange:367-474,antigenLength:753,antigenImageFileName:PA5-52291_CNOT3_O75175-1_Rabbit.svg,antigenImageFileNamePDP:PA5-52291_CNOT3_O75175-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2639956

Product Specific Information

Immunogen sequence: TPAPYAQAVA PPAPSGPSTT QPRPPSVQPS GGGGGGSGGG GSSSSSNSSA GGGAGKQNGA TSYSSVVADS PAEVALSSSG GNNASSQALG PPSGPHNPPP STSKEPSA

Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 83%.

Target Information

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.