Thermo Fisher Scientific Goat anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 680
Alexa Fluor Plus 680으로 결합된 염소 유래 항-닭 IgY (H+L) 2차 항체로, WB 및 ICC/IF에 적합. 교차흡착 처리로 비특이적 결합 최소화. 높은 특이성과 감도로 형광 이미징 및 다중 염색 실험에 최적화.
- 카탈로그번호
- A32934
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.4 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Chicken IgY, Heavy and Light chains |
| Conjugate | Alexa Fluor™ Plus 680 |
| Excitation/Emission Max | 687 / 704 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 6.5 |
| Contains | 0.016% Methylisothiazolone, 0.016% Bromonitrodioxane |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762846 |
| Target | IgY |
| Cross Adsorption | Against chicken serum containing non-immunoglobulin chicken serum proteins |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 633
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Alexa Fluor Plus 800
- AP
- Biotin
- DyLight 350 / 488 / 550 / 594 / 650 / 680 / 755 / 800
- TRITC
- Request custom conjugation available
Product Specific Information
To minimize cross-reactivity, the goat anti-chicken IgY whole antibodies have been affinity-purified and cross-adsorbed against chicken serum containing non-immunoglobulin chicken serum proteins.
Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining.
The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured, and the highly specific secondaries flow through.
This extra purification step enhances performance in multiplexing or multicolor staining experiments (e.g., flow cytometry, tissue/cell fluorescence staining) where cross-reactivity may occur.
Usage Note:
Before use, briefly centrifuge the conjugate solution and use only the supernatant to remove possible aggregates and reduce nonspecific staining.
Dilution should be optimized empirically for each application.
Specificity:
This antibody binds to heavy chains on chicken IgY and light chains on all chicken immunoglobulins. It does not bind non-immunoglobulin chicken serum proteins.
Target Information
Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for chicken immunoglobulins.
They are useful for detection, sorting, or purification of target proteins and provide increased versatility with various detection systems (HRP, AP, fluorescence).
Signal amplification is achieved as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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