
Thermo Fisher Scientific PRKAR1B Monoclonal Antibody (OTI9C5), TrueMAB
Human PRKAR1B 단백질을 인식하는 마우스 단클론 항체로 Western Blot, ICC/IF, Flow Cytometry에 적합. TrueMAB 형식으로 높은 특이성과 재현성을 제공하며, 액상 형태로 -20°C에서 안정적으로 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9C5 |
| Immunogen | Full length human recombinant protein of human PRKAR1B produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.17 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism, and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme, resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified that each represent specific gene products. Elevation of cAMP levels leads to translocation of PKA to the nucleus where it phosphorylates the transcription factor CREB on serine 133, enabling TFIIB binding to TBP1 and linking phospho-CREB to the RNA polymerase II transcription initiation complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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