Thermo Fisher Scientific FICD Polyclonal Antibody
Human FICD 단백질에 특이적인 Rabbit Polyclonal 항체로 Western blot 및 IHC(P) 검증 완료. AMPylation 및 de-AMPylation 관련 단백질 연구에 적합. 항원 친화 크로마토그래피로 정제된 액상 형태로 장기보관 시 -20°C 권장.
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Applications
Western Blot (WB)
- Tested Dilution: 0.04–0.4 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human FICD. Recombinant protein control fragment (Product #RP-90613). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2641634 |
Product Specific Information
Immunogen sequence:
CLAVLKGLYL LRSKPDRAQH AATKCTSPST ELSITSRGAT LLVAKTKASP AGKLEARAAL NQALEMKRQG KREKAQKLFM HALKMDPDFV DALTEFGIFS EEDKDIIQAD YLYTRALTIS PYHEKALVNR DRTLPLVEEI DQRYFSIID
Highest antigen sequence identity to the following orthologs:
- Mouse: 83%
- Rat: 85%
Target Information
Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context.
The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place.
Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed:25601083).
In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at Thr-518, thereby inactivating it.
In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at Thr-518, leading to restore HSPA5/BiP activity.
Although it is able to AMPylate RhoA, Rac, and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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