Thermo Fisher Scientific FICD Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.04–0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Immunogen sequence:
CLAVLKGLYL LRSKPDRAQH AATKCTSPST ELSITSRGAT LLVAKTKASP AGKLEARAAL NQALEMKRQG KREKAQKLFM HALKMDPDFV DALTEFGIFS EEDKDIIQAD YLYTRALTIS PYHEKALVNR DRTLPLVEEI DQRYFSIID

Highest antigen sequence identity to the following orthologs:

• Mouse: 83%
• Rat: 85%

Target Information

Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context.
The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place.
Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed:25601083).
In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at Thr-518, thereby inactivating it.
In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at Thr-518, leading to restore HSPA5/BiP activity.
Although it is able to AMPylate RhoA, Rac, and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.