Thermo Scientific™

BL21(DE3) Competent Cells

Thermo Scientific BL21(DE3) Competent Cells are suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3자세히 알아보기

Thermo Scientific BL21(DE3) Competent Cells are suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG. BL21(DE3) is an E. coli B strain and does not contain the lon protease. It is also deficient in the outer membrane protease OmpT. The lack of these two key proteases reduces degradation of heterologous proteins expressed in the cells.

• High transformation efficiency: >1x107 cfu/μg pUC19 DNA
• Specifically constructed for high-level expression of recombinant proteins
• Genetic markers that minimize RNA and protein degradation
• Convenient two reactions per vial packaging
• S.O.C. Outgrowth medium included

Applications
BL21(DE3) Competent Cells are suitable for high-level T7 expression of recombinant proteins.

Genotype
F– omp_T _hsdSB (rB–, mB–) gal dcm (DE3)

Note: Not suitable for maintenance of expression plasmids. Use DH10B or DH5α competent cells that do not carry the gene for T7 RNA polymerase for plasmid DNA propagation (Cat. Nos. EC0112, EC0113).

사양

제품 유형Competent Cell

효율High Efficiency (>1x10^7 cfu/μg pUC19 DNA)

세포 유형Chemically Competent

메틸화 DNA 클로닝Yes

박테리아 또는 효모 균주BL21(DE3)

블루/화이트 스크리닝No

배송 조건Dry Ice

종E. coli

프로모터LacUV5

형식Tube

제품라인Thermo Scientific™

수량10 x 100 μL

Unit SizeEach