Thermo Scientific™

GeneBLAzer™ RARα-UAS-bla HEK 293T Cells

RAR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor alpha fused to the자세히 알아보기

RAR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor alpha fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK293T cell line. GeneBLAzer™UAS-bla HEK 293T cells (Cat. No. K1104) stably express a beta-lactamase reporter gene under the transcriptional control of a 7X Upstream Activator Sequence (UAS). Transcription from the 7X UAS is activated by the binding of the GAL4 transcription factor DNA-binding-domain (DBD). The GAL4-DBD is expressed as a fusion protein with the ligand binding domain (LBD) of RAR alpha. When an agonist binds to the LBD of the GAL4(DBD)-RAR alpha(LBD) fusion protein it translocates to the nucleus where it binds to the 7X UAS inducing transcription of betalactamase. RAR alpha-UAS-bla HEK 293T cells have been tested for assay performance using variable assay conditions, including DMSO concentration, cell number, stimulation time, substrate loading time and have been validated for Z` and EC50 concentrations of all-trans retinoic acid. Additional testing data using alternate stimuli are also available.

사양

대체 작용제Tetrac, T4

길항제1-850

어세이 입력Cell-based beta lactamase reporter gene

셀 상태Dividing Cells

검출 방법Fluorescence

약효를 기대할 수 있는 타겟Nuclear Receptors

셀 수2 x 106 Cells

일차 작용제T3 Hormone

제품라인GeneBLAzer™

제품 유형HEK 293T Cells

수량2 x 106 cells

리포터 유전자BLA (Beta-Lactamase)

타겟 입력RARA, RAR alpha, NR1B1

Unit SizeEach