Thermo Scientific™

GeneBLAzer™ ERα-UAS-bla GripTite™ Cells

ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding자세히 알아보기

ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla GripTite™ cell line. GeneBLAzer™ UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER alpha-UAS-bla GripTite™ cells are functionally validated for Z` and EC50 concentrations of 17-beta Estradiol. In addition, ER alpha-UAS-bla GripTite™ cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

사양

대체 작용제Betamethasone, Cortisol, Budesonide

길항제Milfepristone, Cortisone, Progesterone

어세이 입력Cell-based beta lactamase reporter gene

셀 상태Dividing Cells

검출 방법Fluorescence

종Human

약효를 기대할 수 있는 타겟Nuclear Receptors

셀 수2 x 106 Cells

일차 작용제Dexamethasone

제품라인GeneBLAzer™

제품 유형GripTite Cells

수량2 x 106 cells

리포터 유전자BLA (Beta-Lactamase)

배송 조건Room Temperature

타겟 입력ER alpha, ESR1, ERA, NR3A1

Unit SizeEach