Thermo Fisher Scientific BAF53A Polyclonal Antibody
Rabbit 폴리클로날 항체로 인간 BAF53A 단백질(25–75번 잔기)을 인식. Western blot, IHC, IP 등 다양한 응용 가능. 액상 형태로 공급되며 4°C 보관. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 | Epitope retrieval with citrate buffer pH 6.0 recommended for FFPE sections |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 25 and 75 of human BRG1-associated factor, 53 kDa subunit A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
BAF53A is a member of the actin-related protein (ARP) family, sharing significant sequence identity with conventional actins. Both actins and ARPs possess an ATP-binding cleft known as the actin fold. ARPs participate in various cellular processes, including vesicular transport, spindle orientation, nuclear migration, and chromatin remodeling.
The BAF53A gene encodes a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex, functionally related to the SWI/SNF complex in yeast and Drosophila. This complex facilitates transcriptional activation by counteracting chromatin-mediated repression. Together with beta-actin, BAF53A is required for maximal ATPase activity of BRG1 and for chromatin/matrix association of the BAF complex. Three transcript variants encoding two different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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