Thermo Fisher Scientific IFN alpha Monoclonal Antibody (RMMA-1)

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Neutralization (Neu)

Assay-dependent

-

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

RMMA-1

Immunogen

mouse interferon alpha

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.5 mg/mL

Purification

Affinity chromatography, Ion-exchange chromatography, Size-exclusion chromatography

Storage buffer

0.3M sodium bicarbonate, pH 8.0, with 0.2M NaCl

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_223544

Product Specific Information

221001 targets IFN alpha in ELISA and Neutralization applications and shows reactivity with mouse samples. Purity is greater than 95%, and endotoxin level is less than 1 EU/ug.

In neutralization assays, one neutralization unit is the amount of antiserum required to neutralize one unit of mouse IFN alpha to a 50% endpoint. This antibody is expected to work in IP applications, although it has not been fully evaluated for IP.

After receipt, the product may be stored at -20C for short-term use (under 6 months). For long-term storage, the product should be kept at -70C or below for retention of full activity.

IFN alpha has a predicted molecular weight of approximately 19 kDa.

Target Information

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.