Thermo Fisher Scientific Ly-6C Monoclonal Antibody (HK1.4), APC, eBioscience
마우스 Ly-6C 항원을 인식하는 HK1.4 단클론 항체로, APC 형광단백질로 결합되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 0.125 µg/test로 사용 가능합니다. 4°C 암소 보관, 동결 금지. 연구용 전용 제품입니다.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.125 µg/test
- Publications: View 117 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2c, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HK1.4 |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1724155 |
Additional Available Formats
- Alexa Fluor 488
- APC-eFluor 780
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-Cyanine5.5
- Super Bright 436
- Custom conjugation available upon request
Product Specific Information
The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family.
Ly-6C is expressed on monocytes/macrophages, endothelial cells, granulocytes, and subsets of lymphocytes.
Expression levels vary among mouse strains:
- Ly6C.1 (C57Bl/6, SJL): stains both CD4 and CD8 cells
- Ly6C.2 (BALB/c, 3H/He): stains CD8 but not CD4 cells
In vitro addition of HK1.4 antibody can increase proliferation and stimulate cytokine release.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested with mouse splenocytes at ≤ 0.125 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Titrate antibody for optimal performance.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Granulocytes are white blood cells characterized by cytoplasmic granules.
Also known as polymorphonuclear leukocytes (PMN or PML), they typically have lobed nuclei.
Neutrophil granulocytes are the most abundant type.
Granulocytes or PMN are released from bone marrow under regulation by complement proteins.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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