Thermo Fisher Scientific MEKK1 Polyclonal Antibody
MEKK1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot과 IHC(P)에서 검증됨. 인간 시료에 반응하며, MAPK 신호전달 연구에 적합. 고순도 정제 및 안정적 저장 조건 제공.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to amino acids 1145–1176 of the C-terminal region of human MEKK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Purification | Ammonium sulfate precipitation, size-exclusion chromatography, dialysis |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C for short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2141424 |
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Target Information
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK).
MEKK1 phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK.
The kinases of these signaling cascades are highly conserved, with homologs in yeast, Drosophila, and mammalian cells.
MEKK1 can phosphorylate and activate MAPKK1 and MAPKK2 (MEK1/MEK2), leading to MAP kinase activation.
It is also a highly efficient activator of the JNK cascade.
The protein contains a putative RING-type zinc finger and a SWIM-type zinc finger.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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