Thermo Fisher Scientific ACOT2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 222-483 of human ACOT2 (NP_006812.3). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ACOT2,uniProtId:P49753-1,ncbiNodeId:9606,antigenRange:222-483,antigenLength:483,antigenImageFileName:PA5-120270_ACOT2_P49753-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120270_ACOT2_P49753-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.79 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913842

Product Specific Information

Positive test controls include: 293T, LO2, U-87MG, Mouse kidney. The target is usually found in the following locations: Mitochondrion.

Immunogen sequence: IVDMFGTGGG LLEYRASLLA GKGFAVMALA YYNYEDLPKT METLHLEYFE EAMNYLLSHP EVKGPGVGLL GISKGGELCL SMASFLKGIT AAVVINGSVA NVGGTLHYKG ETLPPVGVNR NRIKVTKDGY ADIVDVLNSP LEGPDQKSFI PVERAESTFL FLVGQDDHNW KSEFYANEAC KRLQAHGRRK PQIICYPETG HYIEPPYFPL CRASLHALVG SPIIWGGEPR AHAMAQVDAW KQLQTFFHKH LGGHEGTIPS KV

Target Information

Acyl-CoA thioesterases (ACOTs) are a group of enzymes that catalyze the hydrolysis of acyl-CoA to form coenzyme A (CoA) and a free fatty acid. Through their catalytic activity, ACOTs are able to regulate the level of fatty acids and acyl-CoAs within the cell. ACOT1 (acyl-CoA thioesterase 1, also known as CTE1) and ACOT2 (acyl-CoA thioesterase 2, also known as PTE2) are members of the ACOT family and exhibit different cellular localization, with ACOT1 existing as a monomer in the cytoplasm and ACOT2 localized primarily to mitochondria. Characteristic of most ACOT proteins, ACOT1 and ACOT2 catalyze the conversion of Palmitoyl-CoA and water to free CoA and palmitate, a reaction that is important for the regulation of intercellular fatty acid levels. ACOT2 is expressed as multiple alternatively spliced isoforms and, like ACOT1, is encoded by a gene which maps to human chromosome 14.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.