Thermo Fisher Scientific VIM Monoclonal Antibody (OTI1H10), TrueMAB
Human Vimentin 단백질(aa 210-466)을 항원으로 한 Mouse monoclonal antibody. Western blot에 최적화되어 있으며, 1:200~1:1,000 희석 사용 권장. 액상 형태로 1 mg/mL 농도, PBS/BSA/glycerol buffer에 보관. -20°C에서 보관, 반복 동결 해동은 피해야 함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 210–466 of human VIM produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific IFPs. It also plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin leads to filament breakdown and cytoskeletal rearrangements that facilitate cholesterol transport to mitochondria, enhancing steroid synthesis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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