Himedia TC-100 Insect Medium w/ L-Glutamine w/o Sodium bicarbonate

TC-100 is a modification done by Dr. Gardiner and Dr. Stockdale of the original formulation of Graces insect medium. Sucrose, fructose and several Krebs cycle intermediates were omitted from the original formula. The modification was done to optimize the production of Autographia californica NPV virions by cells from the fall armyworm, Spodoptera frugiperda. IM007 contains L-glutamine and does not require supplementation with insect hemolymph. Instead, tryptose broth and fetal bovine serum provide the necessary growth factors. This medium supports the growth of several lepidopteran cell lines including Spodoptera frugiperda. It is specifically used for production of baculoviruses in lepidopteran cell lines.