Thermo Fisher Scientific Goat anti-Mouse IgG2a Secondary Antibody, HRP

Applications

Tested Dilution

Western Blot (WB)

1:500-1:5,000

Immunohistochemistry (IHC)

1:500-1:2,000

ELISA (ELISA)

1:500-1:2,000

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Mouse IgG2a

Conjugate

HRP HRP HRP

View additional formats

• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Mouse IgG2a/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 555 ](/antibody/secondary/product-name/Mouse IgG2a/conjugates/Alexa Fluor Plus 555/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Mouse IgG2a/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [Alexa Fluor Plus 680 ](/antibody/secondary/product-name/Mouse IgG2a/conjugates/Alexa Fluor Plus 680/host/Goat/)
• [Alexa Fluor Plus 800 ](/antibody/secondary/product-name/Mouse IgG2a/conjugates/Alexa Fluor Plus 800/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG2a/conjugates/Biotin/host/Goat/)
• [PE-Cyanine5.5](/antibody/secondary/product-name/Mouse IgG2a/conjugates/PE-Cyanine5.5/host/Goat/)
• Request custom conjugation

Form

Lyophilized

Purification

Affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2534065

Target

IgG2a

Antibody Form

Whole Antibody

Product Specific Information

Storage and reconstitution: The formulation of this antibody was updated from PBS with 0.02% thimerosal to PBS without thimerosal on 31/Mar/2024, any lots produced after this date will not contain thimerosal. Lot numbers greater than 2855945 will not have thimerosal.

Store product at -20°C until opened. Reconstitute using 200 µL PBS, pH 7.2, to yield a 1 mg/mL stock solution. After reconstitution in buffer without a stabilizer like thimerosal, the product is stable for approximately three months at 4°C. For prolonged storage after reconstitution in buffers without a stabilizer, add glycerol to a final concentration of 50% (v/v), aliquot, and store at -20°C. The antibody will be stable for up to one year stored in this manner.

Alternatively, if the product is reconstituted in a buffer containing a preservative, for example, in PBS, pH 7.2, containing 0.02% thimerosal, the product will be stable for up to one year when stored at 4°C.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.