Invitrogen™

EnzChek™ Direct Phospholipase C Assay Kit

This kit provides continuous monitoring of phosphotidyl choline specific phospholipase C (PLC) activity in microplate based biochemical assays. The assay자세히 알아보기

This kit provides continuous monitoring of phosphotidyl choline specific phospholipase C (PLC) activity in microplate based biochemical assays. The assay uses a PCL selective fluorogenic substrate that emits a bright green emission upon cleavage.

The EnzChek™ Direct Phospholipase C Assay Kit provides a simple and robust method for monitoring PC-PLC activity. Each kit provides enough reagents for 2 microplates, using 200 μl volumes in 96 well format. PC-PLC plays a crucial role in many cell signaling pathways involved in apoptosis and cell survival, as well as diseases ranging from cancer to HIV1-7. The assay uses a glycerophospho-ethanolamine with a dye-labeled sn-2 acyl chain as a substrate for PC-PLC. Substrate cleavage by PC-PLC before the phosphate releases the dye-labeled diacylglycerol, which produces a positive fluorescence signal that may be measured continuously. The reaction product has absorption and fluorescence emission maxima of 509 nm and 516 nm, respectively. Using purified enzyme from Bacillus cereus, the assay can detect as little as 10 mU⁄mL PC-PLC after one hour at room temperature. The kit has been proven useful for characterizing PC-PLC inhibition, and since it offers a direct measurement, the potential for false positives in a compound screen is eliminated.

사양

색상Green

검출 방법Fluorescence

용도(애플리케이션)Phospholipase Assay

용도 (장비)Microplate Reader

제품 유형Phospholipase Assay

염료 유형BODIPY™ FL

방출509⁄516

필터 유형FITC (Fluorescein) Filter

제품라인EnzChek™, Molecular Probes™

수량1 kit

배송 조건Room Temperature

기질 특성Lipid-Based Substrate, Chemical Substrate

기질 유형Phospholipase Substrate

타겟 효소Phosphatidylcholine-Specific Phospholipase C

Unit SizeEach