Thermo Fisher Scientific DDX50 Polyclonal Antibody
Human DDX50 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC(P), ICC/IF에 적합합니다. Antigen affinity chromatography로 정제되었으며, PBS/glycerol buffer에 보존됩니다. 연구용으로 RNA 헬리케이스 관련 연구에 활용됩니다.
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Thermo Fisher Scientific DDX50 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human DDX50. Recombinant protein control fragment (Product #RP-97021). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2640479 |
Product Specific Information
Immunogen sequence:
LSSNAVSQIT RMCLLKGNMG VCFDVPTTES ERLQAEWHDS DWILSVPAKL PEIEEYYDGN TSSNSRQRSG WSSG
Highest antigen sequence identity to orthologs:
- Mouse: 93%
- Rat: 95%
Target Information
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in various cellular processes that alter RNA secondary structure, including translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.
DDX50 is a DEAD box enzyme possibly involved in ribosomal RNA synthesis or processing. DDX50 and DDX21 (RH-II/GuA) have similar genomic structures and are tandemly oriented on chromosome 10, suggesting gene duplication during evolution. The DDX50 gene has pseudogenes on chromosomes 2, 3, and 4. Alternative splicing generates multiple transcript variants, though only one full-length variant is defined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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