Invitrogen™

pcDNA™3.4 TOPO™ TA Cloning Kit

The pcDNA™ 3.4 TOPO™ vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.자세히 알아보기

The pcDNA™ 3.4 TOPO™ vector is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression. It can be used in suspension-adapted cells, such as Expi293F™, FreeStyle™ 293-F, and FreeStyle™ CHO-S cells, for transient protein expression. The vector can also be used as a Geneticin™-selectable expression plasmid for engineering stable cell lines. The enhanced, full-length CMV promoter and other expression elements typically enable higher expression levels than pcDNA™ 3.1-, 3.2-, and 3.3-based expression constructs.

The pcDNA 3.4 TOPO™ vector contains the following elements:

• Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
• WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
• TOPO™ cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
• Neomycin resistance gene for selection of stable cell lines with Geneticin™
• pUC origin for high copy replication and maintenance of the plasmid in E. coli

The pcDNA3.4™ TOPO™ TA Cloning Kit includes TOPO™-adapted plasmid vector, reagents for PCR cloning of your gene of choice into the vector, a sequencing primer for confirmation of insert orientation, and One Shot™ TOP10 competent cells.

사양

항생제 내성 박테리아Ampicillin (AmpR)

클로닝 방법TOPO™-TA

구성 또는 유도성 시스템Constitutive

배달 유형Transfection

용도(애플리케이션)Protein Production, Constitutive Expression

플라스미드High Copy Plasmid

제품라인TOPO™

제품 유형TA Cloning Kit

프로모터CMV

단백질 태그Untagged

수량20 μg Kit

복제 개시점pUC Origin

선택 제제(진핵)Geneticin™ (G-418)

선택 마커 진핵NeoR

선택 마커 프로모터SV40 Promoter

벡터pcDNA

Unit SizeEach