Thermo Fisher Scientific NFIA Monoclonal Antibody (OTI6F1), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

NF-1 (also known as CTF) is a family of CCAAT box-binding proteins that stimulate DNA replication and activate transcription.
Human NF-1 mRNA analysis reveals two isoforms arising from alternative splicing of a single gene. NF-1 binds its consensus DNA element as a homodimer through an N-terminal DNA-binding domain and activates transcription via a proline-rich C-terminal transactivation domain.
NF-1 recognizes the adenovirus type 2 promoter and activates transcription of herpes simplex virus thymidine kinase genes.
The NF-1 consensus element is found in promoter regions of various eukaryotic genes, including Ha-Ras, α-globin, HSP70, GRP78, Histone H1, myelin basic protein, and Xenopus laevis vitellogenin gene promoter.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.