Thermo Fisher Scientific Human ABL1 [E255K], His Tag Recombinant Protein

Applications

• Kinase Assay (KA): Assay-dependent

Product Specifications

Product Specific Information

• The mutation was confirmed by LC-MS/MS using digestion with Glu-C.
• For maximum recovery, please spin prior to use.
• Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use directly from original packaging.
• For larger sizes, aliquot to minimize freeze/thaw cycles (aliquots below 20 µL not recommended).
• Do not store diluted kinase.
• Properly stored at -80°C, product is guaranteed for 6 months from date of purchase.
• Protein Form: Full Length

Target Information

c-Abl (Abelson murine leukemia viral oncogene homolog 1, ABL1) is a 140 kDa proto-oncogene member of the Src family of non-receptor tyrosine kinases. It plays roles in cell differentiation, division, adhesion, and stress response.
Activity is negatively regulated by its SH3 domain; deletion of SH3 converts c-Abl into an oncogene. The t(9;22) translocation fuses BCR and c-Abl genes, common in chronic myelogenous leukemia (CML).
c-Abl’s DNA-binding activity is regulated by CDC2-mediated phosphorylation, suggesting a role in the cell cycle.
The c-Abl oncogene is implicated in several human leukemias, including:

• 90–95% of chronic myelocytic leukemia (CML)
• 20–25% of adult acute lymphoblastic leukemia (ALL)
• 2–5% of pediatric ALL

c-Abl localizes to dynamic actin structures and phosphorylates CRK, CRKL, DOK1, and other proteins involved in cytoskeleton dynamics. It may also regulate DNA repair by triggering proapoptotic pathways when DNA damage is irreparable.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.