Thermo Fisher Scientific UBE3B Polyclonal Antibody
Human UBE3B 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용에 사용 가능. Affinity chromatography로 정제된 고순도 항체이며, PBS/glycerol buffer에 보관. 단백질 유비퀴틴화 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human UBE3B (Accession Q7Z3V4), corresponding to amino acid residues R981–P1031 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2818667 |
Product Specific Information
Antibody detects endogenous levels of total UBE3B.
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes:
- E1 ubiquitin-activating enzymes
- E2 ubiquitin-conjugating enzymes
- E3 ubiquitin-protein ligases
This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates.
A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases.
Shorter isoforms lacking the C-terminal HECT domain are unlikely to bind E2 enzymes.
Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: PA5-99734_UBE3B_Q7Z3V4-1_Rabbit.svg, PA5-99734_UBE3B_Q7Z3V4-1_Rabbit_PDP.jpeg)
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