Thermo Fisher Scientific ergo-1 Polyclonal Antibody, Biotin
C. elegans의 ERGO-1 단백질을 인식하는 Biotin 결합 다클론 항체. Western blot, ELISA, IP 등에 사용 가능. 친화 크로마토그래피로 정제되었으며, 안정적 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | C. elegans |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide taken within amino acid region 790–840 on C. elegans endogenous-RNAi deficient argonaute (ERGO-1) protein. |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Argonaute protein required for gene silencing in the endogenous RNA interference (RNAi) pathway. Involved in the 26G RNAi pathway and associates with both unmethylated and methylated 26G small interfering RNAs (26G-siRNAs), which are a class of 26 nucleotide siRNAs possessing a guanine residue at the 5′-end. Associated 26G-siRNAs are methylated by the methyltransferase henn-1, which stabilizes the siRNAs. Association with 26G-siRNAs is required for the biogenesis of secondary 22G-siRNAs (22 nucleotide siRNAs with a triphosphorylated guanine residue at the 5′-end). May be involved in passenger strand cleavage of target 26G-siRNAs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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