Thermo Fisher Scientific CHGA Monoclonal Antibody (OTI2E12), TrueMAB
CHGA 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC, ICC/IF에 사용 가능. 인간 및 생쥐 반응성. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용. 신경내분비 종양 마커 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:4000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E12 |
| Immunogen | Full length human recombinant protein of human CHGA produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Chromogranin A (CHGA) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins.
It is found in secretory vesicles of neurons and endocrine cells and serves as a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin.
These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system.
CHGA is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors.
Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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