Thermo Fisher Scientific Choline Oxidase Polyclonal Antibody, HRP
Choline Oxidase를 인식하는 HRP 결합 폴리클로날 항체로 Western blot, ELISA, IP에 적합. Alcaligenes 유래 항원을 면역원으로 사용. 정제된 염기 교환 크로마토그래피 공정으로 제조되어 높은 특이성과 재현성 제공. 연구용 전용 시약.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:1,000–1:2,500 |
| Immunoprecipitation (IP) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Choline Oxidase [Alcaligenes species] |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15 M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration. For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only before immediate use.
Anti-Choline Oxidase antibody is an IgG fraction purified from monospecific antiserum through delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis.
Immunoelectrophoresis shows a single precipitin arc against anti-Peroxidase, anti-Goat Serum, and purified Choline Oxidase [Alcaligenes species].
Cross-reactivity with Choline Oxidase from other sources is unknown.
Target Information
Choline Oxidase catalyzes the reaction:
Choline + 2 O₂ + H₂O → Betaine + 2 H₂O₂
This enzyme participates in the first step of the subpathway synthesizing betaine from choline.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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