Thermo Fisher Scientific H2AK5ac Polyclonal Antibody
H2AK5ac에 특이적인 Rabbit Polyclonal Antibody로, Western blot, ELISA, ChIP, ChIP-Seq 등에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS buffer에 보관. 인간 시료에 반응하며 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:25,000 |
| ChIP assay (ChIP) | 0.5–1 µg |
| ChIP-sequencing (ChIP-Seq) | Assay-dependent |
| Peptide Array (Array) | 1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to the human histone H2A region containing acetylated lysine 5 (H2AK5ac). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.93 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609563 |
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules each of H2A, H2B, H3, and H4 form an octamer around which approximately 146 bp of DNA is wrapped, forming nucleosomes. The linker histone H1 interacts with linker DNA and contributes to chromatin compaction into higher-order structures. The gene encoding this histone is intronless, lacks a polyA tail, and contains a palindromic termination element. It is located within the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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