Thermo Fisher Scientific GFAP Polyclonal Antibody
GFAP 단백질을 인식하는 Thermo Fisher Scientific의 goat polyclonal antibody로, 인간·마우스·랫트에 반응합니다. Western blot 및 IHC 등 다양한 응용에 적합하며, 고순도 액상 형태로 제공됩니다. 신경교세포 마커 연구에 유용합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.001–0.1 µg/mL | – |
| Immunohistochemistry (IHC) | – | 2 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | 1 publication |
| Immunocytochemistry (ICC/IF) | – | 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (DGEVIKESKQEHKD) corresponding to the C-terminus amino acids of GFAP (aa 417–430) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10984384 |
Product Specific Information
- Predicted to react with canine and rat based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
GFAP (Glial fibrillary acidic protein) is a class III intermediate filament protein specifically expressed in astrocytes and certain astroglia of the central nervous system. It is also found in satellite cells of peripheral ganglia and non-myelinating Schwann cells. Neural stem cells strongly express GFAP, making it a key astrocytic marker.
GFAP expression is elevated in various brain tumors derived from astrocytic cells and can also be found in lens epithelium, Kupffer cells, salivary tumors, and erythrocytes.
GFAP is used to distinguish astrocytes from other glial cells during development. Mutations in the GFAP gene cause Alexander disease, a rare astrocytic disorder.
Alternative splicing results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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