Thermo Fisher Scientific P4HA1 Polyclonal Antibody
Western blot, ICC/IF, ELISA에 적합한 P4HA1 단클론 항체로, Human, Mouse, Rat 반응성을 가집니다. Rabbit에서 유래한 Polyclonal IgG로, Affinity Chromatography로 정제되었으며 PBS/glycerol buffer에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 235–534 of human P4HA1 (NP_001017962.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.02 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804548 |
Product Specific Information
- Positive Samples: U-87MG, LO2, A-431, 293T
- Cellular Location: Endoplasmic reticulum lumen
Immunogen sequence:
DPEHQRANGN LKYFEYIMAK EKDVNKSASD DQSDQKTTPK KKGVAVDYLP ERQKYEMLCR GEGIKMTPRR QKKLFCRYHD GNRNPKFILA PAKQEDEWDK PRIIRFHDII SDAEIEIVKD LAKPRLRRAT ISNPITGDLE TVHYRISKSA WLSGYENPVV SRINMRIQDL TGLDVSTAEE LQVANYGVGG QYEPHFDFAR KDEPDAFKEL GTGNRIATWL FYMSDVSAGG ATVFPEVGAS VWPKKGTAVF WYNLFASGEG DYSTRHAACP VLVGNKWVSN KWLHERGQEF RRPCTLSELE
Target Information
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline essential for proper folding of procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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