Thermo Fisher Scientific 6 His Polyclonal Antibody
6x His 태그 단백질 검출용 염소 폴리클로날 항체. Western blot, ELISA, ICC/IF, IP 등 다양한 응용에 적합. 1 mg/mL 액상 형태로 제공되며, Protein A 정제 및 PBS 완충액에 보존. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:400 |
| ELISA | 1:10,000–1:30,000 |
| Immunoprecipitation (IP) | 1–4 µg/mg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | 6-His (HHHHHH) conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either the N- or C-terminal region of recombinant proteins to enable purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins even when specific antibodies are not available. His-tagged proteins are purified through metal chelate chromatography due to the histidine cluster’s affinity for metal ions such as Ni²⁺.
His tag-specific antibodies facilitate detection and co-immunoprecipitation of His-tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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